CAMP test

The CAMP test is used to differentiate between beta-hemolytic streptococci. It allows us to distinguish Streptococcus agalactiae from Streptococcus pyogenes. S. aureus and Streptococcus agalactiae or Streptococcus pyogenes lines are crossed on blood agar . Each of these three bacteria causes beta-hemolysis.

Overview of substances produced by individual bacteria: S. aureus produces beta-hemolysin and Streptococcus agalactiae produces the so-called CAMP factor. Streptococcus pyogenes does not produce CAMP factor.

If we cross the S. aureus line with the Streptococcus agalactiae line , the substances they produce will interact - beta-hemolysin and CAMP factor will interact. This interaction causes the hemolysis zone to enlarge . A bow-shaped clarification occurs at the site of interaction. Thus, the interaction of Streptococcus pyogenes and S. aurea does not, of course, increase the hemolysis zone.

The name is derived from the names of the discoverers - Christie, Atkins, Munch-Petersen.

Results:

  • positive – Streptococcus agalactiae,
  • negative – Streptococcus pyogenes.


  • Positive result of the culture CAMP test. In this case, Streptococcus agalactiae . Vertical inoculation line Staphyloccus aureus .
    Positive result of the culture CAMP test. In this case, Streptococcus agalactiae . Vertical inoculation line Staphyloccus aureus .
  • Negative CAMP test result. Test strain Streptococcus pyogenes , inoculation line Staphylococcus aureus .
    Negative CAMP test result. Test strain Streptococcus pyogenes , inoculation line Staphylococcus aureus .
  • CAMP test inverse result . Test strain Rhodococcus equi . Vertical inoculation line Staphylococcus aureus .
    CAMP test inverse result . Test strain Rhodococcus equi . Vertical inoculation line Staphylococcus aureus .


  • Bacitracin test
  • Catalase test
  • Plasma coagulase test
  • Optochin test

References

  • ŠMÍROVÁ, Václava. Introduction to medical microbiology. - edition. -. 
  • BEDNÁŘ, M, V FRAŇKOVÁ and J SCHINDLER, et al. Medical microbiology - bacteriology, virology, parasitology. 1st edition. Prague: Marvil, 1996. 558 pp.  ISBN 80-238-0297-6 .
  • TANKESHWAR ACHARYA,. CAMP Test: Principle, Procedure and results: CAMP test  [online]. © 2013. [feeling. 2017-05-17]. < https://microbeonline.com/camp-test-principle-procedure-results/ >.