Microbial picture of the vagina

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Vaginal microbiological examination (MPV) is one of the ways of determining a number of sexually transmitted diseases. Secretion is collected with a sterile cotton swab using speculums from the mucous membrane of vagina a from cervix. The material is transferred to two glass slides and stained according to:

  1. Gram – diagnostics of bacteria and yeast;
  2. Giemsa – diagnosis of the presence of trichomonads.
MOP Evaluation Discharge Microscopic image Note
MOP I MOP of healthy women No discharge It can be demonstrated only in a part of clinically completely healthy women
MOP II Non-purulent microbial discharge-bacterial vaginosis Milky cloudy, sometimes yellowish; variously thick and viscous consistency
  • No or minimal leukocyt
  • The amount of bacteria (most commonly Gardnerella vaginalis )
  • Lactobacilli are completely absent or only sporadic
The presence of so-called "clue cells - " epithelial cells of the vaginal mucosa, to which a number of different bacteria adhere
MOP III Purulent bacterial discharge Thick whitish to yellowish discharge
  • A large number of polymorphonuclears
  • A large number of different bacteria
  • Lactobacilli are usually absent
  • Relatively few epithelia
Pyogenic bacteria are mostly responsible - coliform rods, streptococci, staphylococci, enterococci, ...
MOP IV Acute or chronicgonorrhoea Thick yellow-white to yellow-green discharge
  • Acute stage – almost exclusively leukocytes with intra- and extraleukocyte-localized G-diplococci that look like a coffee bean
  • Chronic stage - also admixture of other bacteria and a small amount of epithelia, the predominance of leukocytes is not as pronounced as in the acute phase
Evaluation of the finding is difficult, the final diagnosis is based on repeated culture or PCR examination.
MOP V Trichomoniasis (Trichomonas vaginalis) Thin, white, often foamy
  • Trichomonas vaginalis
  • Epithelia, leukocytes
  • Also lactobacilli and a mixture of different bacteria
Stained according to Giemsa – Trichomonad cells often disintegrate, only violet-red nuclei can be observed (usually pointed in one place) surrounded by remnants of bluish cytoplasm
MOP VI Vaginal candidosis Various thick whitish discharge
  • Yeast (saprophytic and parasitic phase)
  • Leukocytes may or may not be present
  • Lactobacilli and other bacteria
Overgrowth due to hormonal fluctuations, weakened immunity (AIDS), antibiotic treatment, diabetes, etc.

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  • ONDROVČÍK, Petr – VOTAVA, Miroslav. Selected chapters in clinical microbiology. 1. edition. Masarykova univerzita v Brně, 1998. 90 pp. ISBN 80-210-1805-4.