Burri dyeing

Cryptococcus neoformans





This method allows a negative representation of bacterial envelopes .

Method
  1. Apply the bacteria to a slide in a drop of ink ;
  2. using the second slide, spread the drop evenly over the entire surface, waiting for it to dry;
  3. repaint with crystal violet .

The bacteria appear purple, the background black and the mucous membrane does not stain .

  • Gram stain
  • Giemsa staining

Source

  • RYŠKOVÁ, Olga, et al. Instructions for practical exercises in medical microbiology. 1st edition. Prague: Karolinum, 1997.  ISBN 80-7184-307-5 .