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Brucella spp.
Brucella melitensis on blood agar
Morphology For cocobacilli
Relation to oxygen strictly aerobic
Cultivation prolonged blood agar
Antigens surface L antigen
Source domestic and wild animals
Transmission contact with a sick animal, contaminated aerosol and milk
Incubation time 2-4 weeks
Disease brucellosis
Diagnostics blood culture, cultivation, serology
Therapy doxycycline in combination with rifampicin
MeSH ID D002002

Brucella bacteria are small, gram-negative, strictly aerobic immobile short rods or cocobacilli. They do not form spores. These are typical animal parasites. B. abortus , B. melitensis , B. suis and B. canis are pathogenic to humans .

Brucella spp.

Cultivation and biochemistry[edit | edit source]

Brucella require an extended cultivation time . Colonies have a glossy moist appearance, can be translucent to cloudy and often show fluorescence. They are catalase positive and oxidase positive. Bacteria produce endotoxin , which in its effects resembles enterobacterial endotoxin .

Disease[edit | edit source]

See the Brucellosis page for more information .

Brucellosis is a typical anthropozoonosis , the infection is typical in both domestic and wild animals. The site of infection in humans is most often injured skin or conjunctiva, and infection can occur by inhaling a contaminated aerosol or consuming improperly treated milk from sick animals. Thanks to a careful veterinary inspection, brucellosis has been eradicated in the Czech Republic; today, only imported diseases occur in our country .

Brucellas are among the intracellular parasites of phagocytic cells , in macrophages they are distributed throughout the body.  Granulomas subsequently form in the affected organs. The course of the disease varies from species to brucella.

Diagnostics[edit | edit source]

Diagnosis is more complicated due to non-specific clinical manifestations, it is most often performed using blood culture and serologically. In antibody-based assays, brucellosis show cross-reactivity with Francisella tularensis . The most reliable method of diagnosis is cultivation, which is often not successful due to its complexity.

Therapy[edit | edit source]

The combination of doxycycline with rifampicin is given for six weeks. In children, doxycklin is replaced by co-trimoxazole.

Links[edit | edit source]

related articles[edit | edit source]

Reference[edit | edit source]

  1. ↑Jump up to:a b c d VOTAVA, Miroslav, et al. Medical microbiology special. 1st edition. Brno: Neptun, 2003. 495 pp.  ISBN 80-902896-6-5 .
  2. ↑Jump up to:a b c BENEŠ, Jiří, et al. Infectious medicine. 1st edition. Galén, 2009. 651 pp.  ISBN 978-80-7262-644-1 .
G +
Micrococcus Micrococcus luteus
Rhodococcus Rhodococcus equi
facultatively anaerobic
Enterococcus Enterococcus duransEnterococcus faecalisEnterococcus faecium
Streptococcus Streptococcus agalactiaeStreptococcus mutansStreptococcus pneumoniaeStreptococcus pyogenesStreptococcus suisOral streptococci
Staphylococcus Staphylococcus aureusStaphylococcus epidermidisStaphylococcus intermediusStaphylococcus saprophyticus
Peptococcus Peptococcus niger
Peptostreptococcus Peptostreptococcus anaerobiusPeptostreptococcus prevotiiPeptostreptococcus vaginalis
aerobic + facultative anaerobic
Arcanobacter Arcanobacterium haemolyticum
Bacillus Bacillus anthracisBacillus cereus
Corynebacterium Corynebacterium diphtheriaeCorynebacterium jeikeiumCorynebacterium ulceransCorynebacterium urealyticum
Erysipelothrix Erysipelothrix rhusiopathiae
Listeria Listeria monocytogenes
Nocardia Nocardia asteroidesNocardia brasiliensis
Rhodococcus Rhodococcus equi
Actinomyces Actinomyces israeli • Actinomyces naeslundi
Bifidobacterium Bifidobacterium dentium
Clostridium Clostridium botulinumClostridium difficileClostridium novyiClostridium tetaniClostridium perfringensClostridium septicumClostridium ulcerans
Lactobacillus Lactobacillus acidophilus
Propionibacterium Propionibacterium acnesPropionibacterium propionicus
Acinetobacter Acinetobacter calcoaceticus
Moraxella Moraxella catarrhalisMoraxella lacunata
Neisseria Neisseria gonorrhoeaeNeisseria meningitidis • Non-pathogenic species of Neisseria
Veillonella Veillonella alcalescensVeillonella parvula
Rickettsia Rickettsia prowazekiiRickettsia rickettsiiRickettsia typhi
Alcaligentes Alkaligentes feacalis
Bartonella Bartonella bacilliformisBartonella henselaeBartonella quintana
Bordetella Bordetella bronchisepticaBordetella parapertussisBordetella pertussis
Brucella Brucella abortusBrucella canisBrucella melitensisBrucella suis
Burkholderia Burkholderia cepaciaBurkholderia malleiBurkholderia pseudomallei
Francisella Francisella tularensis
Legionella Legionella pneumophila
Kingella Kingella denitrificansKingella kingaeKingella oralis
Pseudomonas Pseudomonas aeruginosaPseudomonas fluorescens
Stenotrophomonas Stenotrophomonas maltophilia
facultatively anaerobic
Actinobacillus Actinobacillus equliActinobacillus lignieresii
Aeromonas Aermonas caviaeAeromonas hydrophilaAeromonas sobria
Afipia Afipia felis
Citrobacter Citrobacter freundiCitrobacter koseri
Eikenella Eikenella corrodens
Enterobacter Enterobacter aerogenesEnterobacter cloacae
Escherichia Escherichia coli
Haemophilus Haemophilus ducreyiHaemophilus haemolyticusHaemophilus influenzaeHaemophilus parainfluenzae
Klebsiella Klebsiella granulomatisKlebsiella oxytocaKlebsiella pneumoniae
Pasteurella Pasteurella haemolyticaPasteurella multocidaPasteurella ureae
Plesiomonas Plesiomonas shigelloides
Proteus Proteus mirabilisProteus vulgaris
Salmonella Salmonella Enteritidis • Salmonella Typhi • Salmonella Paratyphi
Serratia Serratia marcescens
Shigella Shigella boydiiShigella dysenteriaeShigella flexneriShigella sonnei
Vibrio Vibrio choleraeVibrio parahemolyticus
Yersinia Yersinia enterocoliticaYersinia pestisYersinia pseudotuberculosis
Campylobacter Campylobacter coliCampylobacter fetusCampylobacter jejuni
Helicobacter Helicobacter pylori
Bacteroides Bacteroides fragilisBacteroides vulgatus
Fusobacterium Fusobacterium necrophorumFusobacterium nucleatumFusobacterium stabile
Leptotricha Leptotricha buccalis
Mobiluncus Mobiluncus curtisiiMobiluncus mulieris
Prevotella Prevotella melaninogenica
Porphyromonas Porphyromonas gingivalis
acid resistant
Mycobacterium Atypical mycobacteriaMycobacterium tuberculosisMycobacterium leprae
non-stainable G +/-
strictly aerobic
Leptospira Leptospira biflexaLeptospira interrogansLeptospira parva
Borrelia Borrelia burgdorferiBorrelia hermsiBorrelia recurrentisBorrelia vincenti
strictly anaerobic
Treponema Non-pathogenic treponemsTreponema carateumTreponema pallidumTreponema phagedenisTreponema pertenue
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