OZPP module - Practicum No. 1

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Preparation No. 1 and No. 2 - van Gieson staining (elastic fibers)

Structures

• tunica intima
• tunica media
• tunica adventitia

Preparation no. 3 and no. 4 – myocardium (HE)

Structures

• cardiomyocytes
• box-shaped nuclei of cardiomyocytes

Preparation no. 5 and no. 6 – fat identification (liver)

Structures

• intracytoplasmic fat vacuoles in hepatocytes

Preparation no. 7 and no. 8 – silvering according to Grocott (proof of mold)

Preparation no. 9 and no. 10 – blue trichrome staining (kidney)

Structures

• collagen fiber

Preparation no. 11 and no. 12 – van Gieson staining (gallbladder)

Structures

• smooth muscle
• ligament

Preparation no. 13 and no. 14 – staining for reticular fibers (liver)

Structures

• reticular fibers

Preparation no. 15 and no. 16 – staining according to Mowry (appendix vermiformis)

Structures

• lymphatic follicle with a germinal center
• intracytoplasmic mucus vacuoles in mucosal lining cells

Preparation no. 17 and no. 18 – PAS staining (Grawitz's approx.)

Structures

• PAS positive glycogen granules in the cytoplasm of tumor cells

Preparation no. 19 and no. 20 – detection of iron by Perls reaction (lungs)

Structures

• alveoli
• positive siderophages in the iron test according to Perls

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