Cerebrospinal fluid examination

From WikiLectures

Cerebrospinal fluid testing is one of the basic methods that contribute to the diagnosis of neurological diseases. The cerebrospinal fluid is most often collected by lumbar puncture (3×5 ml, between L4–L5 or S1), the suboccipital approach is less common. The cerebrospinal fluid needs to be transported to the laboratory as quickly as possible, as the cells gradually break down, the glucose concentration decreases and the lactate increases.

The basic examination of cerebrospinal fluid includes the performance of these analyzes
Searchtool right.svg For more information see Cerebrospinal fluid spectrophotometry.

Further examination of cerebrospinal fluid includes these determinations
  • determination of IgG, IgA, IgM, albumin in plasma and cerebrospinal fluid with estimation of intrathecal immunoglobulin synthesis and determination of blood-brain barrier disorder,
  • isoelectric focusing for the detection of IgG oligoclonal bands,
  • some other tests (e.g. determination of other proteins in cerebrospinal fluid and serum, specially stained cytological preparations).
Searchtool right.svg For more information see Proteins in cerebrospinal fluid.

Cerebrospinal fluid is mainly examined in these diseases

Contraindications to consumption
  • finding of intracranial expansion process,
  • Intracranial hypertension,
  • local infection at the puncture site,
  • some coagulopathies,
  • we take 1–2 ml separately due to artificial blood contamination.

Links[edit | edit source]

Related articles[edit | edit source]

External links[edit | edit source]

Lumbar puncture – instructional video

  • FIALOVÁ, L. – VEJRAŽKA, M. Základní vyšetření mozkomíšního moku [online]. ©2005. The last revision 2008, [cit. 8. 9. 2009]. <https://el.lf1.cuni.cz>.

Použitá Literatura[edit | edit source]

  • ADAM, P. Cytologie mozkomíšního moku (CD-ROM). 1. edition. SEKK, 2000. 
  • AMBLER, Z – BEDNAŘÍK, J – RŮŽIČKA, E. Klinická neurologie – část obecná. 1. edition. Triton, 2004. ISBN 80-7254-556-6.
  • GLOSOVÁ, L. Cytologický atlas mozkomíšního moku. 1. edition. Galén, 1998. ISBN 80-85824-70-1.
  • KALA, M. – MAREŠ, J. Lumbální punkce a mozkomíšní mok. 1. edition. Galén, 2008. ISBN 978-80-7262-568-0.
  • MASOPUST, J. Klinická biochemie. Požadování a hodnocení biochemických vyšetření I. a II. část. 1. edition. Karolinum, 1998. ISBN 80-7184-650-3.
  • NEVŠÍMALOVÁ, S – RŮŽIČKA, E – TICHÝ, J. Neurologie. 1. edition. Galén, 2005. ISBN 80-7262-160-2.
  • SCHNEIDERKA, Petr. Kapitoly z klinické biochemie. 2. edition. Karolinum, 2004. ISBN 80-246-0678-X.
  • RACEK, J. Klinická biochemie. 1. edition. Galén – Karolinum, 1999. ISBN 80-7262-023-1.
  • ŠTERN, P. Obecná a klinická biochemie pro bakalářské obory studia. 1. edition. Karolinum, 2005. ISBN 978-80-246-1025-2.
  • ZIMA, T. Laboratorní diagnostika. 1. edition. Galén – Karolinum, 2002. ISBN 80-7262-201-3.