Amylase/stool determination

From WikiLectures

Determination of pancreatic amylase in faeces [1][2][3][4] is a relatively little used test, which is also an immunochemical test with an antibody to human pancreatic amylase - a novelty that appeared in 2000. Normal values are above 360 µg / g stool, calibration standards are in the range of 0-12,000 µg / g. The test is based on the ILMA (ImmunoLuminoMetric Assay) principle with a luminescent measurement marker being acridinium ester. The original work on the determination of pancreatic amylase in stool mainly describes the detection of isoenzymes and the correlation with other pancreatic enzymes in stool in patients with pancreatic insufficiency.

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  1. MORIYOSHI, Yuriko, Tadashi TAKEUCHI a Keiko SHIRATORI. Fecal isoamylase activity in patients with pancreatic diseases. Pancreas. 1991, vol. 6, no. 1, s. 70-76, ISSN 0885-3177.
  2. GROMASHEVSKAIA, LL, GF STREMETSKII a TV BOGATYR'. Opredelenie izofermentov amilazy v kale. Laboratornoe delo. 1981, roč. 1981, no. 10, s. 609-612, ISSN 0023-6748. 
  3. MÜLLER, G. Fakale Enzymdiagnostik. Deutsche Zeitschrift für Verdauungs- und Stoffwechselkrankheiten. 1979, vol. 39, no. 6, s. 257-265, ISSN 0012-1053. 
  4. HINTNER, I, G MÜLLER a A THEUNE. Bestimmungen von Isoenzymen der alkalischen Phosphatase und alpha-Amylase im Stuhl. Zeitschrift für die gesamte innere Medizin und ihre Grenzgebiete. 1977, roč. 15, vol. 32, no. 12, s. 292-295, ISSN 0044-2542.