Tetramethylbenzidine is used as a chromogen in detection systems based on the peroxidase and pseudoperoxidase reactions. In fact, in the presence of a suitable catalyst (peroxidase, complex-bound iron of haemoglobin or myoglobin), colourless tetramethylbenzidine is oxidised with hydrogen peroxide (or other hydroperoxide, e.g. cumene hydroperoxide) to benzidine blue:
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Bibliography[edit | edit source]
- National Center for Biotechnology Information. PubChem Compound [online]. [cit. 2009-10-09]. <https://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=41206&loc=ec_rcs>.