Nitrotetrazolium blue

Reduction of nitrotetrazolium blue (NBT) to colored formazan

Nitroblue tetrazolium (NBT) is used to detect reducing substances. Reduction of tetrazolium rings in NBT leads to their opening, forming formazan^[1]. In doing so, the absorption spectrum of the substance changes and the originally light yellow color changes to dark blue. The reaction is suitable for spectrophotometric determination.

NBT is a fairly widely used reagent. It is used, for example, to determine glycated proteins, a fairly typical application is also the determination of superoxide production in various systems.

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↑ {{#switch: article |book = Incomplete publication citation. BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK1980. pp. 830-833. Also available from <https://pubs.acs.org/action/cookieAbsent>. |collection = Incomplete citation of contribution in proceedings. BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK. 1980. pp. 830-833. Also available from <https://pubs.acs.org/action/cookieAbsent>. {{ #if: |978-80-7262-438-6} } |article = BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK. Reduction of nitro blue tetrazolium by CO2- and O2- radicals. 1980, year 1980, well. 8, pp. 830-833, also available from <https://pubs.acs.org/action/cookieAbsent>. ISSN 1089-5639. DOI: 10.1021/j100445a006. |web = Incomplete site citation. BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK. ©1980. <https://pubs.acs.org/action/cookieAbsent>. |cd = Incomplete carrier citation. BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK. ©1980. |db = Incomplete database citation. ©1980. <https://pubs.acs.org/action/cookieAbsent>. |corporate_literature = Incomplete citation of company literature. BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK. 1980. Also available from <https://pubs.acs.org/action/cookieAbsent>. legislative_document = Incomplete citation of legislative document. 1980. s. 830-833. Also available from URL <https://pubs.acs.org/action/cookieAbsent>. ISSN 1089-5639.

[1] {{#switch: article |book = Incomplete publication citation. BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK1980. pp. 830-833. Also available from <https://pubs.acs.org/action/cookieAbsent>. |collection = Incomplete citation of contribution in proceedings. BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK. 1980. pp. 830-833. Also available from <https://pubs.acs.org/action/cookieAbsent>. {{ #if: |978-80-7262-438-6} } |article = BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK. Reduction of nitro blue tetrazolium by CO2- and O2- radicals. 1980, year 1980, well. 8, pp. 830-833, also available from <https://pubs.acs.org/action/cookieAbsent>. ISSN 1089-5639. DOI: 10.1021/j100445a006. |web = Incomplete site citation. BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK. ©1980. <https://pubs.acs.org/action/cookieAbsent>. |cd = Incomplete carrier citation. BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK. ©1980. |db = Incomplete database citation. ©1980. <https://pubs.acs.org/action/cookieAbsent>. |corporate_literature = Incomplete citation of company literature. BIELSKI, Benon HJ, Grace G SHIUE and Stanley BAJUK. 1980. Also available from <https://pubs.acs.org/action/cookieAbsent>. legislative_document = Incomplete citation of legislative document. 1980. s. 830-833. Also available from URL <https://pubs.acs.org/action/cookieAbsent>. ISSN 1089-5639.

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