Nitrotetrazolium blue

Nitroblue tetrazolium (NBT) is used to detect reducing substances. Reduction of tetrazolium rings in NBT leads to their opening, forming formazan^[1]. In doing so, the absorption spectrum of the substance changes and the originally light yellow color changes to dark blue. The reaction is suitable for spectrophotometric determination.

NBT is a fairly widely used reagent. It is used, for example, to determine glycated proteins, a fairly typical application is also the determination of superoxide production in various systems.

Links

References

↑ BIELSKI, Benon HJ – SHIUE, Grace G – BAJUK, Stanley. Reduction of nitro blue tetrazolium by CO2- and O2- radicals. Journal of physical chemistry [online]. 1980, vol. 84, no. 8, p. 830-833, Available from <https://pubs.acs.org/action/cookieAbsent>. ISSN 1089-5639. DOI: 10.1021/j100445a006.

[1] BIELSKI, Benon HJ – SHIUE, Grace G – BAJUK, Stanley. Reduction of nitro blue tetrazolium by CO2- and O2- radicals. Journal of physical chemistry [online]. 1980, vol. 84, no. 8, p. 830-833, Available from <https://pubs.acs.org/action/cookieAbsent>. ISSN 1089-5639. DOI: 10.1021/j100445a006.

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