Minimum inhibitory concentration
The minimum inhibitory concentration (MIC) is the smallest concentration of antimicrobial substances , which inhibits visible growth of microorganism. An increase / inhibition readout can only be carried out when it occurs in a control well (hole with inoculated broth without the addition of an antimicrobial substance) to turbidity, ie the observable growth of bacteria. Template:Dobrý příklad
Method[edit | edit source]
- The strain is inoculated into the wells of the microtiter plates with the medium, where the added concentration increases in a row of antibiotic;
- We incubate until checking (usually overnight) increases
- We observe turbidity or sediment - from the first hole, where growth is already suppressed and the medium remained clean, reading the MIC.