Minimum inhibitory concentration

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500px-MIC mikrobiologie.png

The minimum inhibitory concentration (MIC) is the smallest concentration of antimicrobial substances , which inhibits visible growth of microorganism. An increase / inhibition readout can only be carried out when it occurs in a control well (hole with inoculated broth without the addition of an antimicrobial substance) to turbidity, ie the observable growth of bacteria. Template:Dobrý příklad

Method[edit | edit source]

  1. The strain is inoculated into the wells of the microtiter plates with the medium, where the added concentration increases in a row of antibiotic;
  2. We incubate until checking (usually overnight) increases
  3. We observe turbidity or sediment - from the first hole, where growth is already suppressed and the medium remained clean, reading the MIC.

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