Fetal membranes (SFLT)

Fetal membranes – overview (HE)
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Description: Fetal envelopes include (from fetus to uterus): Aep – amniotic epithelium, EEM – extraembryonic mesoderm, T – trophoblast, D – decidua capsularis, AD – amniotic cavity, filled with amniotic fluid in vivo.

Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!

Fetal membranes – a closer look (HE)
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Description: Fetal envelopes include (from fetus to uterus): Aep – amniotic epithelium, EEM – extraembryonic mesoderm, T – trophoblast, D – decidua capsularis, AD – amniotic cavity, filled with amniotic fluid in vivo.

Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!

Fetal membranes – amnion and extraembryonic mesoderm (HE)
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Description: Aep – amniotic epithelium – one layer of cubic cells, washed in vivo by amniotic fluid (in the amniotic cavity – AD), it lies on a clearly visible basement membrane, under this epithelium is the extraembryonic mesoderm (EEM).

Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!

Extraembryonic mesoderm, trofoblast and decidua (HE)
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Description: EEM – extraembryonic mesoderm, VV – vascular layer of extraembryonic mesoderm (well-perfused layer adjacent to trophoblast), T – trophoblast, D – decidua capsularis. Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!

Fetal membranes – overview (proof of pan-cytokeratin - immunohistochemistry)
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Description: Detection of keratin using antibodies. Keratin is present in cells of epithelial origin.

Thus, amniotic epithelium (Aep) and trophoblast (T) were shown. The fibrous layers – extraembryonic mesoderm (EEM) and decidua (D) – are therefore not highlighted. AD – amniotic cavity, VV – vascular layer of extraembryonic mesoderm (well-perfused layer adjacent to the trophoblast).

Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!

Fetal membranes – amnion (proof of pan-cytokeratin - immunohistochemistry)
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Description: Detection of keratin using antibodies. Keratin is present in cells of epithelial origin.

Thus, the amniotic epithelium (Aep) and not the extraembryonic mesoderm (EEM, ligament) was highlighted. AD – amniotic cavity

Artef. – artifact!! in this bright spot is in vivo EEM, the split occurred during sample collection/processing, so do not confuse with sparse tissue, cavity or adipose tissue !!

Fetal membranes

 * umbilical cord (SFLT)


 * Fetal membranes (SFLT)


 * Placenta (SFLT)


 * Chorion frondosum (SFLT)

Resources

 * Histologický atlas (3. LF UK)