Stanovení bilirubinu v moči

Detection of bilirubin in urine by diagnostic strips is based on the azocoupling reaction provided by conjugated bilirubin with a stable diazonium salt (eg 2,6-dichlorobenzenediazonium tetrafluoroborate ). A pink to pinkish-red dye is formed. When high concentrations of urobilinogen occur, the color changes to orange. In this case, it is recommended to evaluate the coloration up to 2 minutes after the indication zone has wetted. Lower to false negative results may be due to high concentrations of ascorbic acid. Urine specimens should be protected from direct sunlight, which causes oxidation of bilirubin, followed by a falsely inferior to negative finding.

Only conjugated bilirubin is examined in the urine, as unconjugated bilirubin cannot be excreted in it.