Infections caused by Streptococcus agalactiae

Streptococcus agalactiae is a Group B Gram-positive β-hemolytic coccus, often referred to as GBS ("Group B Streptococcus"). It is often a part of the flora of the gastrointestinal and urogenital tract (asymptomatic carrier). During delivery, transmission from a pregnant woman to a newborn is possible. In neonates, GBS is significantly involved in perinatal morbidity and mortality, so in indicated cases, colonized women are given antibiotics during childbirth as a precaution.

In newborns, it causes early and late infections - bacteremia, sepsis, pneumonia and meningitis. In adults (especially with chronic diseases such as diabetes mellitus, etc.), infections manifest themselves in the form of bacteraemia (including sepsis) and soft tissue infections. In pregnant women, GBS causes bacteremia (sepsis), amnionitis and urinary tract infections.

The Czech Gynecological and Obstetrics and Neonatology Society has developed a recommended procedure for GBS screening, antibiotic prophylaxis during childbirth and follow-up care for newborns.

Streptococcus agalactiae v těhotenství

 * recommended screening:
 * in all pregnant women between 35 and 37 weeks of gestation;
 * cultivation from the lower third of the vagina and rectum;
 * the result is recorded in the pregnancy certificate; the examination is valid for 5 weeks;
 * indications for intrapartum antibiotic prophylaxis:
 * positive GBS screening;
 * GBS bacteriuria at any time during pregnancy;
 * previous child with invasive GBS infection in neonatal age;
 * unknown result of GBS screening + risk factors (delivery 37 + 0 gestational weeks, amniotic fluid outflow> 18 hours, maternal febrile);
 * intrapartum antibiotic prophylaxis is not indicated:
 * negative GBS screening (regardless of risk factors);
 * cesarean delivery before the onset of labor and with intact amniotic sacs (regardless of the outcome of the GBS screening);
 * positive GBS screening / bacteriuria in previous pregnancy;
 * intrapartum antibiotic prophylaxis:
 * First choice ATB: penicillin G iv (there is still good sensitivity in the Czech Republic);
 * ATB alternative: ampicillin iv, in case of allergy to penicillin ATB cephalosporins 1st generation iv (cefazolin, cephalothin) or clindamycin iv;
 * ATB therapy is terminated at the birth of the fetus, and is continued only if there is a clear clinical finding of maternal infection.

Infections caused by Streptococcus agalactiae in neonates
Streptococcus agalactiae (GBS) is the main and most common cause of early neonatal infections, significantly contributing to neonatal morbidity and mortality. In an effort to reduce the incidence of early neonatal GBS infections, screening for GBS colonization in pregnant women has been introduced and, in indicated cases, intrapartum antibiotic prophylaxis is performed. Prophylaxis is considered sufficient if antibiotics are given at least 4 hours before the birth of the newborn. After birth, the algorithm developed by the Czech Neonatological Society is followed. According to this algorithm, high-risk newborns are increasingly observed in the first 48 hours of life, or laboratory signs of inflammation are examined.

Newborns are most often colonized by GBS during childbirth. Only a small proportion of colonized newborns develop invasive GBS infections. Risk factors include, in particular, prematurity and premature amniotic fluid outflow.

GBS causes early and late infections in newborns. Early sepsis usually manifests in the first 24 hours, but can occur at any time in the first week of life. The symptoms are the same as in early neonatal sepsis of a different etiology. Pneumonia with bacteremia is also common, meningitis is less common. Late GBS infection can occur at any time between the first week of life and 3 months of age. These are typically bacteremia and meningitis.

Recommendations of the American Academy of Pediatrics (AAP)
Early GBS infection (GBS EOD) is defined as the isolation of group B streptococcus (GBS) from blood, cerebrospinal fluid or other normally sterile sites from birth to day 6 of age.

GBS EOD manifests itself clinically at or shortly after delivery. Most babies are symptomatic within 12 to 24 hours of birth, about 95% of infections are diagnosed within 48 hours of birth.

GBS EOD most often arises from the ascending colonization of the uterine compartment of GBS from the gastrointestinal and urogenital flora of the mother. About 50% of newborn GBS positive mothers are colonized with GBS and about 1-2% develop GBS EOD. Risk factors include: lower gestational age (less effective opsonization and neutrophil-mediated immune response, lower levels of protective antibodies transmitted from the mother), longer amniotic fluid outflow (ascending colonization and infection), maternal fever (evidence of maternal inflammatory response to developing intraamniotic infection). ), a child with GBS EOD at a previous birth (impaired maternal immune response, increased GBS virulence), GBS bacteriuria (a sign of a high degree of maternal colonization). Following intrapartum antibiotic prophylaxis (IAP), recolonization occurs within 24-48 hours after delivery. IAP does not protect against the development of late GBS infection. A woman colonized with GBS during pregnancy has about a 50% risk of colonization in the following pregnancy.
 * Antenatální screening GBS


 * cultivation of swab from vagina and rectum in 36 + 0. - 37 + 6. week of pregnancy or in case of imminent preterm birth and / or amniotic fluid discharge before the 37th week of pregnancy;
 * in GBS bacteriuria, it is not necessary to confirm colonization by culturing a swab from the vagina and rectum;
 * Traditional microbiological or molecular methods can be used to detect GBS - NAAT ( nucleic acid amplification tests ) - however, NAAT does not allow the determination of sensitivity, which is important, for example, in women with allergies to penicillin.
 * Intrapartum antibiotic prophylaxis


 * objective: to reduce the incidence of invasive GBS EOD by interrupting the vertical transmission of GBS (transient reduction of colonization of the mother's vagina, prevention of colonization of the fetus / neonatal surface and mucous membranes, reaching the level of antibiotic in the neonatal blood exceeding MIC - minimum inhibitory concentration)
 * indication:
 * pregnant with positive antenatal screening GBS (positive cultivation from vagina or rectum);
 * women with GBS bacteriuria diagnosed at any time during pregnancy;
 * women who had a child with GBS infection in a previous pregnancy;
 * women with premature birth and / or premature amniotic fluid before 37 + 0. week of gestation;
 * women giving birth at 37 + 0. week of gestation with unknown GBS status and risk factors during labor (maternal temperature at labor ≥ 38 ° C, amniotic fluid outflow ≥ 18 hours;
 * NAAT positive for GBS;
 * antibiotics:
 * beta-lactam antibiotics: penicillin G (crosses the placenta and is excreted by the fetal kidneys into amniotic fluid), ampicillin (broader antimicrobial spectrum than penicillin);
 * in case of penicillin allergy with low risk of anaphylaxis: cefazolin;
 * in allergy to penicillin with a high risk of anaphylaxis: clindamicin (examine sensitivity - increasing resistance of GBS to clindamicin; metabolized fetal liver, poorly excreted in the amniotic fluid, reaches significant concentrations only after multiple doses);
 * in penicillin allergy with high risk of anaphylaxis and clindamicin-resistant GBS: vancomycin;
 * GBS EOD risk assessment

In the United States, the incidence of early GBS infection has decreased significantly following the introduction of antenatal screening and intrapartum antibiotic prophylaxis of GBS. Meningitis was diagnosed in 9.5% of neonates with GBS EOD. GBS meningitis demonstrated by positive cerebrospinal fluid culture was accompanied by a negative blood culture in 9.1% (ie the incidence of these cases was about 2.5 cases per 1 million live births). GBS infections account for about 45% of all cases of early infection (EOD) with positive blood culture in full-term neonates and about 25% in very low birth weight infants. 28% of GBS EOD cases are neonates born before 37 weeks of gestation. GBS EOD mortality is 2.1% in preterm infants and 19.2% in preterm infants (<37 weeks of gestation).

Late GBS infection (GBS LOD)

is defined as isolation of GBS from day 7 to day 89 of life from a normally sterile site. Rarely, very late GBS infections may occur after 3 months of age, especially in very premature infants or in children with immunodeficiency. The incidence of GBS LOD did not decrease after the introduction of intrapartum antibiotic prophylaxis. The most common form of GBS LOD is bacteremia without focus. Another form of GBS LOD is meningitis, bone and joint infections or isolation of GBS from peritoneal fluid are relatively rare.

Related articles

 * Streptococcal infections
 * Streptococcus agalactiae