Burker chamber

Bürker's chamber is a special glass slide. It is used when determining the number of red or white blood cells.

https://www.wikiskripta.eu/images/c/c4/Burkerova_komurka.JPG

Dimensions of Bürker's chamber

 * Depth  is 0,1 mm.


 *  The grid  is formed by a system of small squares (with side a=0.05 mm, the volume of the space bounded by this type of square is therefore 1/4000 mm 3 ) and large squares (with side a=0.2 mm, the volume of the space bounded by this type of square is i.e. 1/250 mm 3 ).

Principles of using the Bürker chamber

 * Drop the Blood onto the edge of the coverslip with a Pasteur pipette and let it flow into the chamber (do not drip directly into the chamber!).


 * When determining the number of erythrocytes, we subtract the number in 80 small squares..


 * When determining the number of leukocytes, we subtract the number in 50 large squares.

Related articles

 * Determination of the number of cells, their viability and metabolic activity

Source

 * Institute of Pathological Physiology of the Faculty of Medicine, Charles University in Pilsen
 * Own notes on practical exercises in Physiology, Institute of Physiology 1.LF UK in Prague, winter semester 2013/2014