Oxidase test

Oxidase test (also known as the Kovacs test) is used to determine bacteria, especially in urogenital infections. Gram-negative bacteria, which produce some types of cytochrome c oxidase, give a positive result. Oxidase-positive includes, for example, Pseudomonas aeruginosa, Pasteurella multocida, Vibrio spp., Aeromonas spp. or Neisseria spp.. Oxidase-negative ,on the other hand, includes E. coli, Klebsiella pneumoniae, Enterobacter cloacae, Serratia spp. and Acinetobacter spp..

The oxidase assay uses a redox reagent that is colorless in the reduced form and intensely colored in the oxidized form (usually blue). The agent can be applied to bacterial colonies in liquid form. More commonly, diagnostic strips impregnated with reagent are applied to the bacterial colony.

Source

 * ws:Oxidázový test