Cytological examination of cerebrospinal fluid

Cytological examination of cerebrospinal fluid is part of the basic examination of cerebrospinal fluid. It contributes to the diagnosis of neuroinfections, bleeding into CNS, autoimmune diseases or tumors. Includes:
 * 1) quantitative cytology - consists in determining the number of cellular elements,
 * 2) qualitative cytology - cell types in cerebrospinal fluid and their number are determined.

Method of determination

 * Native non-centrifuged cerebrospinal fluid should be used for cytological examination and elemental counting within 3 hours of collection (0.1 ml is sufficient). Stained with acid fuchsin.
 * Cells are counted in a Fuchs-Rosenthal chamber having a volume of approximately 3 mm3; Therefore, the results are expressed as a fraction of n/3. Erythrocytes are read in the unstained specimen, the other elements are counted in the stained specimen.

Rating

 * We find up to 10/3 (3 in 1 ml) in the cerebrospinal fluid of an adult, the value 11–15/3 is considered borderline. The normal number of cells is referred to as oligocytosis, with an increasing number of cells we speak of pleocytosis.
 * In a qualitative evaluation, only lymphocytes (60-80%) and monocytes (20-40%) are present in the normal cerebrospinal fluid, rarely ependymal cells or chorioid plexus cells.
 * The presence of erythrocytes may be due to artificial injury to the vessel during lumbar puncture or as a result of bleeding into the cerebrospinal fluid.
 * The presence of other elements (granulocytes, activated lymphocytes, plasma cells, activated monocytes, tumor cells) is also a symptom of pathological processes in the CNS.

Pleocytosis

 * Monocytic
 * Monocyte cells predominate and their activated forms are increasing. They represent a non-specific reaction to previous irritation of the nervous system (eg CNS ischemia, terminal phase of inflammation with a clearing reaction, condition after CNS angiography).


 * Granulocytic
 * A marked increase in neutrophilic granulocytes (thousands to tens of thousands) is typical of purulent (bacterial) inflammations. The predominance of eosinophils occurs in allergic reactions or in some neuroinfections (parasitic, fungal).


 * Lymphocytic
 * Pleocytosis with a predominance of lymphocytes, including activated forms, is a characteristic finding for non-purulent inflammatory diseases of viral origin, but sometimes in some bacterial neuroinfections.

Pathological oligocytosis
(the total number of elements does not exceed the norm, their representation is pathological)
 * Monocytic
 * It is characterized by a predominance of monocytes and an increased relative proportion of activated monocytes. This non-specific pattern may be accompanied, for example, by bleeding into the cerebrospinal fluid, where erythrocyte-absorbed macrophages or a terminal phase of inflammation may be detected.


 * Granulocytic
 * Granulocyte oligocytosis with a predominance of neutrophils is a common finding in incipient purulent and non-purulent neuroinfections.


 * Lymphocytic
 * It is characterized by a predominance of lymphocytes with an increased relative proportion of activated forms. The presence of plasma cells indicates intrathecal antibody synthesis. It is typical of chronic neuroinfections and for multiple sclerosis.


 * Tumorous pleocytosis or oligocytosis
 * Tumor elements in the cerebrospinal fluid originate in tumors located near the cerebrospinal fluid pathways or in the malignant infiltration of the meninges.

Related Articles

 * Cerebrospinal fluid
 * Biochemical examination of cerebrospinal fluid
 * Proteins in cerebrospinal fluid
 * Cerebrospinal fluid spectrophotometry
 * Cerebrospinal fluid syndromes

Used literature

 * ADAM, P, et al. Cytologie mozkomíšního moku (CD-ROM). 1. vydání. Praha : SEKK, 2000.


 * AMBLER, Z, J BEDNAŘÍK a E RŮŽIČKA. Klinická neurologie – část obecná. 1. vydání. Praha : Triton, 2004.  ISBN 80-7254-556-6.


 * GLOSOVÁ, L. Cytologický atlas mozkomíšního moku. 1. vydání. Praha : Galén, 1998.  ISBN 80-85824-70-1.


 * KALA, M. a J MAREŠ. Lumbální punkce a mozkomíšní mok. 1. vydání. Praha : Galén, 2008.  ISBN 978-80-7262-568-0.


 * MASOPUST, J. Klinická biochemie. Požadování a hodnocení biochemických vyšetření I. a II. část. 1. vydání. Praha : Karolinum, 1998.  ISBN 80-7184-650-3.


 * NEVŠÍMALOVÁ, S, E RŮŽIČKA a J TICHÝ, et al. Neurologie. 1. vydání. Praha : Galén, 2005.  ISBN 80-7262-160-2.


 * SCHNEIDERKA, Petr, et al. Kapitoly z klinické biochemie. 2. vydání. Praha : Karolinum, 2004.  ISBN 80-246-0678-X.


 * RACEK, J, et al. Klinická biochemie. První vydání. Praha : Galén – Karolinum, 1999. s. 317.  ISBN 80-7262-023-1.


 * ŠTERN, P, et al. Obecná a klinická biochemie pro bakalářské obory studia. 1. vydání. Praha : Karolinum, 2005.  ISBN 978-80-246-1025-2.


 * ZIMA, T, et al. Laboratorní diagnostika. 1. vydání. Praha : Galén – Karolinum, 2002. s. 728.  ISBN 80-7262-201-3.