Spectrophotometry

A number of determinations in biochemistry make use of the fact that many substances absorb electromagnetic radiation in the visible or ultraviolet part of the spectrum, less often infrared radiation. The extent to which a substance absorbs light of different wavelengths (ie, the absorption spectrum) depends on the structure of the compound. The amount of light of a certain wavelength that is absorbed by, for example, a substance dissolved in a solution depends on the concentration of the substance. The measurement of light absorption by a sample is among the most widely used techniques in biochemistry and is referred to as photometry (when measured at one or more specific wavelengths) and spectrophotometry(if measured over a certain continuous range of light wavelengths).

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 * MALDI TOF