Minimum inhibitory concentration

The minimum inhibitory concentration (MIC) is the smallest concentration of antimicrobial substances, which inhibits visible growth of microorganism. An increase / inhibition readout can only be carried out when it occurs in a control well (hole with inoculated broth without the addition of an antimicrobial substance) to turbidity, ie the observable growth of bacteria.

Method

 * 1) The strain is inoculated into the wells of the microtiter plates with the medium, where the added concentration increases in a row of antibiotic;
 * 2) We incubate until checking (usually overnight) increases
 * 3) We observe turbidity or sediment - from the first hole, where growth is already suppressed and the medium remained clean, reading the MIC.

Related articles

 * Antibiotics
 * Incubation time
 * Minimal bactericidal concentration
 * E-test
 * Disc diffusion test