Recombinant DNA


 * Recombinant DNA is an artificially produced DNA.
 * It is usually formed by more DNA sources from the different donor.
 * The sticky ends can put together the individual strands of the DNA.
 * The restriction endonucleases cut the DNA out and DNA ligase incorporates it.
 * For the recombination we need many and many copies of the DNA. That is the reason for using the PCR – polymerase chain reaction.
 * To incorporate the sample od the DNA into the host cell it is necessary to use the vector.
 * After replication in the host cell – on the issue culture – we can work with the exact part of the DNA.
 * The recombinant DNA is much used in the gene manipulation. Thanks to it we are able to produce some important human hormones and enzymes.
 * The clones od the DNA samples can be also used for the forming of the gene maps.

The Process of the DNA Recombination

 * 1) to isolate the exact part of the DNA from the organism
 * 2) to put the DNA into the DNA vector (E.coli, bacteria, yeast)
 * 3) to transfer the vector by into the host
 * 4) to find out the compati sticky ends of two DNA strands
 * 5) to put it together by DNA ligase
 * 6) to clone the new DNA by PCR – polymerase chain reaction
 * 7) to get many copies of the recombinant DNA

Related articles

 * Vector
 * PCR
 * DNA ligase
 * Gene Manipulation