Identification of restriction fragments

Identification of fragments is carried out using so-called samples. A probe is a short natural or artificial DNA of known sequence, labeled with radioactive or fluorescent ethidium bromide. Electrophorogram of the fragments (the so-called "coating") is transferred to a nitrocellulose membrane (blotting), the DNA is denatured and poured over with a sample solution. The monitored fragment associates with the probe (base pairing) and after washing off the "coating" is autoradiographically or fluorescence identified among hundreds of other fragments.

Sequencing methods are among genetic engineering methods. It serves to clarify the structure of the entire fragment (the sample usually represents only a small section of the fragment chain).

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