Neovascularization

__BEZOBSAHU__ Neovascularization je termín pro novotvorbu cév. It is mainly used in the sense of neovasularization of choroidei vessels či novotvorby cév u nádorového bujení.

Neovascularization in oncogenesis
Tumor cells begin to suffer from hypoxií at a deposit size of 1–2 mm3. They then begin to release angiogenic growth factors and suppress angiogenic inhibitor levels. Neovascularization begins with: At this stage, there is also the possibility of the first metastáz.
 * deposit sizes 1–2 mm3;
 * tumor cell count 106;
 * tumor weight 1 mg.

The mechanism of neovascularization is the same as for cells stimulated with growth factors.

náhled|vpravo|Mechanismus angiogeneze

Tumor cells produce: 1) activators
 * VEGFs (vascular endothelial growth factors),
 * FGFs (fibroblast growth factors),
 * PDGF (platelet derived growth factor),
 * EGF (epidermal growth factor),

2) inhibitors
 * thrombospondin-1,
 * statiny – angiostatin, endostatin, canstatin, tumstatin.

Factor synthesis is shifted in favor of activator synthesis. In neovascularization, it is mainly the action of these factors on the precursors, from which the endothelial cells are then activated and eventually the formation of blood vessels takes place.

The role of proangiogenic onkogenes is important in factor synthesis. Oncogene KRas, HRas ® upregulation of VEGF, downregulation of TSP-1 oncogene Fos, Bcl2 ® VEGF expression.

The network of newly formed vessels is irregular, the vessels branch a lot, "twist".

VEGF and related receptors have kinase activity, stimulating angiogenesis, lymphangiogenesis and cell proliferation.

Use of the mechanisms of neovascularization
Thanks to the known mechanism of neovascularization, there is now an effort to develop a way of treating tumors through the cessation of their vascularization. This would cause the cancer cells to become hypoxic and die. The mechanism can be:
 * indirect – inhibition of angiogenic protein synthesis in tumor cells (FGF-β, VEGF, TGF-α)
 * direct – inhibition of endothelial cell response to angiogenic proteins (FGF-β, VEGF, IL-8, PDGF)
 * growth factor receptor inhibition – Pertuzumab, Trastuzumab
 * growth factor inhibition – Bevacizumab (vazba na VEGF ® nenaváže se na receptor)
 * inhibition of receptor kinsaes
 * inhibition of Ras, CDK (kalmodulin dependentní kináza); PKC (proteinkináza C); COX-2 (cyklooxygenáza 2)

Therapy
Bevacizumab
 * This substance is contained in Avastin. It is an anti-VEGF antibody. The drug is given every two weeks through infusions. Avastin is indicated for renal, gastrointestinal, breast, mediastinal, lung and pleural cancers.

Related Articles

 * Biology of oncogenesis
 * Tumor Microenvironment